A Two-level Factorial Design for Screening Factors that Influence the Growth of Staphylococcus sp. strain Amr-15 on Acrylamide

Due to the fact that it breaks down into acrylamide over time, polyacrylamide is one of most important sources in soil. As a strategy for bioremediation, breakdown by action microbes has seen gradual but consistent increase attention all world. A previously isolated molybdenum-reducing bacterium with amide-degrading capability was further identified on significant parameters contributing optimized growth using two-level factorial design this study. The adopted screening five independent factors influencing acrylamide. These include pH, temperature, incubation concentration and glucose concentration. two-factor successful finding acrylamide, which were concentration, pH time (p<0.05) can be RSM future works. or analysed ANOVA, Pareto’s chart perturbations plot other diagnostic plots. plots such as half-normal, Cook’s distance, residual vs runs, leverage Box-Cox, DFFITS, DFBETAS supported conclusion. This study carried out an range well within reported tolerated acrylamide-degrading microorganisms. Incubation expected result since longer allows more ranging from two days been many Most microorganisms grow near-neutral conditions, results obtained conforms published literature trends.